Optimized IVT Platforms: Advancing RNA Quality and Scalability for Animal Biologics Innovation

Part # PS596

Abstract

Hélène Boyer1, Laurence Delaurière1, Dana Brecklin-Benassi1, Daniela Torres Campana1, Laura Alexander1, Maria Dashek1 1Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711

Fidelity 2X Master Mix, ‘RNA’ was generated by in vitro transcription, and ‘ssDNA’ corresponds to reverse transcription output. Automation streamlines production, reduces hands-on time, and ensures reproducibility in process development. An automated method has been developed on the CyBio FeliX Liquid Handler from Analytik Jena, enabling the automation of the synthesis of RNA from a linear DNA input. Figure 8. Automated synthesis of RNA from a linear DNA input. Schematic representation of the automated workflow. Linear DNA is used as input. In vitro transcription is carried out using the RiboMAX Large Scale RNA Production System T7 (automated dispensing of the reaction mix onto the linear input plate). The synthesized RNA is cleaned-up using the ProNex® Size-Selective Purification System. In the rapidly evolving field of animal biologics development, producing high- quality RNA is essential for developing next-generation vaccines and therapeutic interventions. Advanced in vitro transcription (IVT) methodologies provide researchers and process engineers with scalable options to generate large quantities of high-integrity RNA. From efficient transcription systems to precise clean-up and automated workflows, these approaches address the rigorous demands of animal health applications by minimizing immunogenic risk and ensuring consistent results.

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