Protein Expression in Less Time A Short Induction Protocol for KRX

Lynn Litterer and Trista Schagat
Promega Corporation
Publication Date: 2007

Abstract

Single Step (KRX) Competent Cells are ideal for rapidly generating new clones for recombinant protein expression. We examined the effect of induction time and media on recombinant protein yield and activity in KRX. Firefly and Renilla luciferases were used as models. Induction times as brief as 4 hours were sufficient to produce significant quantities of active protein. Using Single Step (KRX) Competent Cells with this short induction protocol, protein can be expressed and analyzed in less time, without the need for overnight induction.

Promega Notes 96, 20–21.