HDAC-Glo™ I/II Assay Technical Manual
Instructions for Use of Product(s)
G6420, G6421, G6422
Literature # TM335
The HDAC-Glo™ I/II Assay is a single-reagent-addition, homogeneous, luminescent assay that measures the relative activity of histone deacetylase (HDAC) class I and II enzymes from cells, extracts or purified enzyme sources. This assay is broadly useful for class I and II enzymes, but sensitivity and performance will vary with catalytic efficiency of particular class I and II isoenzymes. The assay uses an acetylated, live-cell-permeant, luminogenic peptide substrate that can be deacetylated by HDAC activities. Deacetylation of the peptide substrate is measured using a coupled enzymatic system in which a protease in the Developer Reagent cleaves the peptide from aminoluciferin, which is quantified in a reaction using Ultra-Glo™ Recombinant Luciferase. The HDAC-mediated luminescent signal is persistent and proportional to deacetlyase activity, allowing batch processing of multiwell plates.
Summary of Changes
The following changes were made to the 7/25 revision of this document:
1. Removed discontinued products Cat.# G6430, G6431, G6560 and G6570 and any associated usage instructions.
2. Retitled the document HDAC-Glo™ I/II Assay from HDAC-Glo™ I/II Assay and Screening System, and deleted any
mention of the Screening System.
3. Updated Section 6.
4. Removed expired licensing and disclaimer.
5. Updated the cover page, and replaced document font.
Revised 7/25.