pGEM®-T Easy Vector Systems
Convenience and Flexibility for Cloning PCR Products
- Three options for removing the insert with a single digest (BstZI, EcoRI or NotI )
- Ligation can be completed in 1 hour at room temperature
- Available with or without competent cells
Catalog Number:
Size
Add competent cells
Catalog Number: A1360
Catalog Number: A1380
PCR Cloning with Blue/White Selection and Easy Insert Excision
The pGEM®-T Easy Vector Systems are convenient systems to clone PCR products generated by certain thermostable polymerases. These polymerases often add a single deoxyadenosine, in a template-independent fashion, to the 3´-ends of the amplified fragments. The pGEM®-T Easy pre-linearized Vector contains 3´-T overhangs at the insertion site to provide a compatible overhang for PCR products. The provided 2X Rapid Ligation Buffer allows reactions to be completed in 1 hour at room temperature. The incubation period may be extended to increase the number of colonies after transformation. Â
T7 and SP6 RNA polymerase promoters flank a multiple cloning region within the α-peptide coding region for β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be directly identified by Blue/White Screening on indicator plates. The vector allows preparation of single-stranded DNA due to its f1 Origin of Replication.
The pGEM®-T Easy Vector Systems offer all of the advantages of the pGEM®-T Vector Systems with the added convenience of recognition sites for BstZI, EcoRI and NotI flanking the insertion site. Thus, several options exist to remove the desired insert DNA with a single restriction digestion.
The pGEM®-T Easy Vector System II contains JM109 Competent Cells in addition to all of the pGEM®-T Easy Vector System I components.
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
pGEM®-T Easy Vector |
A137A | 1 × 1.2μg | 50ng/μl | |
|
Control Insert DNA |
A363A | 1 × 12μl | ||
|
2X Rapid Ligation Buffer |
C671A | 1 × 200μl | View Product | |
|
T4 DNA Ligase |
M180A | 1 × 100u |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
pGEM®-T Easy Vector |
A137A | 1 × 1.2μg | 50ng/μl | |
|
Control Insert DNA |
A363A | 1 × 12μl | ||
|
T4 DNA Ligase |
M180A | 1 × 100u | ||
|
2X Rapid Ligation Buffer |
C671A | 1 × 200μl | View Product | |
|
JM109 Competent Cells, High Efficiency |
L200A | 6 × 200μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
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