Nano-Glo® Luciferase Assay System
Add-Mix-Measure Assay for NanoLuc® Luciferase
- Bright NanoLuc® reporter enables use in applications where sensitivity is limited
- Add-and-read simplicity allows scaling from bench to HTS
- Extended bright light output optimized for batch and continuous processing
Catalog Number:
Size
Catalog Number: N1110
Catalog Number: N1120
Catalog Number: N1130
Catalog Number: N1150
What is the Nano-Glo® Luciferase Assay System?
The Nano-Glo® Luciferase Assay System is a dedicated detection reagent for NanoLuc® Luciferase, a 19.1kDa monomeric enzyme engineered from the deep-sea shrimp Oplophorus gracilirostris. The system uses the Nano-Glo Luciferase Assay® Substrate to generate bright, ATP-independent, glow-type luminescence with low background, enabling sensitive detection in cell-based assays. It is widely used in molecular biology, cell signaling, gene expression, drug screening, and protein interaction studies.
Learn more about NanoLuc® Luciferase Technology.
How Does the Nano-Glo® Luciferase Assay Work?
The Nano-Glo® Luciferase Assay uses a simple add-and-read protocol. First, combine the Nano-Glo® Luciferase Assay Substrate with Nano-Glo® Luciferase Assay Buffer to create the working reagent. The reagent lyses cells and simultaneously provides the NanoLuc® Luciferase Assay Substrate so no separate lysis step is needed.
When you add the prepared reagent to samples expressing NanoLuc® Luciferase, the luminescent reaction produces a stable light signal. The emitted light has a half-life of approximately 120 minutes in standard tissue culture media, providing a wide measurement window.
You can measure the signal using any standard plate reader or luminometer. Luminescence output is linearly proportional to NanoLuc® Luciferase concentration across a million-fold dynamic range, allowing you to detect both subtle changes and strong expressors.
Simple add-mix-read protocol:
- Add the prepared Nano-Glo® Luciferase Assay Reagent directly to cells or conditioned media.
- Mix briefly.
- Measure luminescence on any standard plate reader or luminometer.
What Are the Benefits of the Nano-Glo® Luciferase Assay?
- High sensitivity: NanoLuc® Luciferase is ~100× brighter than firefly or Renilla luciferase, with low substrate autoluminescence for superior signal-to-background
- Stable glow signal: ~120 min half-life, no injectors needed, ideal for batch processing
- Simple add-mix-read workflow: Integral lysis buffer eliminates separate lysis/injection steps; scales to 1536-well
- Wide dynamic range: Million-fold linear dynamic range
Need to order this product in bulk? Learn about our Custom Manufacturing options.
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
| Nano-Glo® Luciferase Assay Buffer | N112A | 1 × 10ml |
| Nano-Glo® Luciferase Assay Substrate | N113A | 1 × 200μl |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
What's in the box?
| Item | Part # | Size |
|---|---|---|
| Nano-Glo® Luciferase Assay Buffer | N112B | 1 × 100ml |
| Nano-Glo® Luciferase Assay Substrate | N113B | 2 × 1ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
What's in the box?
| Item | Part # | Size |
|---|---|---|
| Nano-Glo® Luciferase Assay Buffer | N112A | 10 × 10ml |
| Nano-Glo® Luciferase Assay Substrate | N113A | 10 × 200μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
What's in the box?
| Item | Part # | Size |
|---|---|---|
| Nano-Glo® Luciferase Assay Buffer | N112B | 10 × 100ml |
| Nano-Glo® Luciferase Assay Substrate | N113C | 5 × 4ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
Frequently Asked Questions
Can I use the Nano-Glo® Luciferase Assay with secreted NanoLuc® Luciferase?
Yes. The Nano-Glo® reagent can be added directly to conditioned culture media to detect secreted NanoLuc® Luciferase without cell lysis. This makes it suitable for monitoring secretion-based reporter assays or for serial sampling of the same culture over time.
Do I need an injector-equipped luminometer to run this assay?
No. The Nano-Glo® Luciferase Assay produces a stable glow-type signal with approximately 120-minute half-life, so there is no need for injectors or rapid-read instrumentation. Any standard microplate reader or luminometer capable of detecting luminescence will work, including the GloMax® Discover System.
How sensitive is the Nano-Glo® Luciferase Assay compared to other luciferase assays?
NanoLuc® Luciferase produces approximately 100-fold greater luminescence intensity than firefly or Renilla luciferase. Combined with the low autoluminescence of the Nano-Glo® Luciferase Assay Substrate, this enables detection of reporter expression at levels where traditional luciferase systems may produce insufficient signal-to-background ratios.
Can I use the Nano-Glo® Luciferase Assay for BRET experiments?
The Nano-Glo® Luciferase Assay System is designed for standard endpoint lytic assays. For BRET and NanoBRET® applications, we offer dedicated NanoBRET® Nano-Glo® Detection Systems optimized for energy transfer measurements with live cells.
Can I use the Nano-Glo® Luciferase Assay for live cell experiments?
The Nano-Glo® Luciferase Assay System is designed for standard endpoint lytic assays. For live-cell NanoLuc® detection without lysis, see the Nano-Glo® Live Cell Assay.
Is the Nano-Glo® Luciferase Assay compatible with high-throughput screening?
Yes. The single-addition, no-injector format and extended signal stability make the Nano-Glo® Assay well suited for batch-mode and continuous-process HTS workflows. The assay scales from individual tubes to 384-well and 1536-well plate formats.
Resources
Articles
- Enhanced extrinsic apoptosis of therapy-induced senescent cancer cells using a death receptor 5 (DR5) selective agonist
- Enhancing anti-EGFRvIII CAR T cell therapy against glioblastoma with a paracrine SIRPγ-derived CD47 blocker
- This study sought to the effect of branched chemically modified poly(A) tails on enhancing mRNA stability and translation capacity.
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