Wizard® SV 96 and SV 9600 Plasmid DNA Purification Systems
Silica Membrane-Based, 96-Well, High-Throughput Plasmid Purification
- 60-minute protocol
- Manual and automated formats
- Vacuum-based method
Catalog Number:
Size
Catalog Number: A2250
Catalog Number: A2255
Catalog Number: A2258
Simple, Reliable Plasmid Purification
The Wizard® SV 96 and SV 9600 Plasmid DNA Purification Systems provide a simple and reliable method for the rapid isolation of plasmid DNA using a silica-membrane, 96-well, high-throughput format. A single plate can be processed in 60 minutes or less. The Vac-Man® 96 Vacuum Manifold eliminates waste handling and allows simultaneous lysate clearing and DNA binding, and the plate design prevents cross-contamination during sample processing.
Purified plasmid can be used directly for automated fluorescent DNA sequencing as well as for other standard molecular biology techniques. Purified DNA meets a target of >98% accuracy over 600 bases using pGEM®-3Zf(+) Vector DNA in BigDye® terminator sequencing.
The Wizard® SV 96 and SV 9600 Systems are designed for use either in a manual format or with Beckman Coulter or PerkinElmer automated instruments.
The Wizard® SV 96 and Wizard® SV 9600 Systems require a vacuum pump capable of generating 15–20 inches of Hg or equivalent and the Vac-Man® 96 Vacuum Manifold (Cat.# A2291) or similar manifold. The Vac-Man® 96 Vacuum Manifold requires the use of an external vacuum trap (not provided).
Learn more about custom options for this product at: www.promega.com/custom/
Protocols
Complete Protocol
Quick Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Alkaline Protease Solution |
A144B | 1 × 3ml | View Product |
|
Wizard® SV 96 Neutralization Solution |
A148D | 1 × 84ml | View Product |
|
Wizard® SV 96 Lysate Clearing Plate |
A224A | 1 × 1 plate | View Product |
|
96-Well Deep Well Plate |
A226A | 1 × 1 plate | |
|
Binding Plate |
A227A | 1 × 1 plate | View Product |
|
Elution Plate |
A228A | 1 × 1 plate | |
|
Plate Sealers |
A230A | 1 × 3 each | |
|
Wizard® SV 96 Cell Resuspension Solution |
A711U | 1 × 40ml | View Product |
|
Wizard® SV 96 Cell Lysis Solution |
A712U | 1 × 40ml | View Product |
|
Nuclease-Free Water |
P119E | 1 × 13ml | View Product |
|
Column Wash Solution (CWA) |
A131C | 1 × 100ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
Column Wash Solution (CWA) |
A1318 | 1 × 370ml | View Product |
|
Alkaline Protease Solution |
A144B | 2 × 3ml | View Product |
|
Wizard® SV 96 Neutralization Solution |
A148E | 1 × 425ml | View Product |
|
Wizard® SV 96 Lysate Clearing Plate |
A224A | 5 × 1 plate | View Product |
|
96-Well Deep Well Plate |
A226A | 5 × 1 plate | |
|
Binding Plate |
A227A | 5 × 1 plate | View Product |
|
Elution Plate |
A228A | 5 × 1 plate | |
|
Plate Sealers |
A230A | 5 × 3 each | |
|
Wizard® SV 96 Cell Resuspension Solution |
A711W | 1 × 125ml | View Product |
|
Wizard® SV 96 Cell Lysis Solution |
A712W | 1 × 125ml | View Product |
|
Nuclease-Free Water |
P119C | 3 × 25ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
Column Wash Solution (CWA) |
A1318 | 20 × 370ml | View Product |
|
Wizard® SV 96 Neutralization Solution |
A1488 | 9 × 950ml | View Product |
|
Wizard® SV 96 Cell Resuspension Solution |
A7118 | 4 × 800ml | View Product |
|
Wizard® SV 96 Cell Lysis Solution |
A7128 | 4 × 800ml | View Product |
|
Wizard® SV 96 Lysate Clearing Plates |
A2248 | 1 × 100 pack | View Product |
|
Wizard® SV 96 Binding Plates |
A2278 | 1 × 100 pack | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
Other Resources
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Frequently Used With
Molecular Biology Lab Guide
A resource designed for scientists just embarking on their career, focusing on fundamental technologies and techniques.
GEN1978
Vac-Man® 96 Vacuum Manifold
For manual use with SV 96 and SV 9600 systems to isolate up to 96 samples simultaneously.
A2291
Nuclease-Free Water
Quality tested and certified free of DNase and RNase activity.
MC1191, P1193, P1195, P1197, P1199
JM109 Competent Cells
E. coli strain ready for transformation; offers blue/white selection.
L2005, L1001, L2001
