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Notes: During brain development endothelial cells may participate in neuronal recruitment, by providing neurotrophic support to new neurons. Levels of  BDNF, NGF, NT-3, and NT-4 mRNAs in cultured adult human brain endothelial cells and human umbilical vascular endothelial cells were determined. The localization of BDNF within these cells was done by immunocytochemistry with the Anti-Human BDNF pAb at a 1:1000 dilution. Cells were fixed with 4% paraformaldehyde with 0.1% glutaraldehyde and permeabilized with  PBS with 0.1% saponin/1% rabbit serum. The Anti-Chicken IgY, HRP Conjugate (1:200 dilution) and a DAB substrate were used to visualize staining. (2392)

Notes: The expression of neurotrophins and their receptors in fetal and adult human bone marrow was examined. Bone marrow smears and sections were fixed and stained for TrkB expression using Promega's Anti-TrkB pAb (1:300 dilution) and the Anti-Chicken Ig,Y HRP Conjugate (1:100 dilution). Frozen and paraffin-embedded fixed human and rat forebrains were stained as positive controls for TrkB expression. (2377)

Notes: The Tfx™-50 Reagent was used to introduce antisense oligonucleotides into osteoclast cells. (1001)

Notes: The antibody was used to detect the expression of BDNF in transfected QT6 quail fibroblasts by immunocytochemistry. The cells were treated with brefeldin prior to assay. (1581)

Notes: The anti-Human BDNF Polyclonal Antibody was used at a 1:200dilution to localize BDNF in primary cultures of rat hippocampal neurons by immunohistochemistry. (1574)