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Int. J. Legal Med. Dec 3, Epub ahead of print. Y-STR DNA analysis of 154 female child sexual assault cases in the Philippines. 2010

Maiquilla, S.M., Salvador, J.M., Calacal, G.C., Sagum, M.S., Dalet, M.R., Delfin, F.C., Tabbada, K.A., Franco, S.A., Perdigon, H.B., Madrid, B.J., Tan, M.P. and De Ungria, M.C.

Notes: The authors used the PowerPlex® Y System to amplify Y-STRs from 154 sexual assault cases in the Philippines. Duplicate amplifications were assembled as recommended by the manufacturer, and amplified products were detected using an ABI PRISM® 310 Genetic Analyzer. Microscopic analysis revealed sperm in 15% of the cases, and Y-STR analysis revealed male DNA in 41% of the cases. (4088)

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Proc. Natl. Acad. Sci. USA 105, 10693–8. Y-chromosomal evidence of a pastoralist migration through Tanzania to southern Africa. 2008

Henn, B.M., Gignoux, C., Lin, A.A., Oefner, P.J., Shen, P., Scozzari, R., Cruciani, F., Tishkoff, S.A., Mountain, J.L. and Underhill, P.A.

Notes: The authors identified a Y-chromosome-specific polymorphism, the M293 mutation, that defines the Y haplotype Eb1f-M293. They examined the geographic distribution of this and other Y haplotypes to determine the date of origin of the E3b1f-M293 haplotype and track the migration of the pastoralist lifestyle in Africa. Y-STR analysis was performed using the PowerPlex® Y System and two additional sets of primers. (4039)

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Forensic Sci. Int. 172, 67–71. Y chromosome haplotypes in Central-South Italy: Implication for reference database. 2007

Rapone, C., Geraci, A., Capelli, C., De Meo, A., D'Errico, G., Barni, F., Berti, A. and Lago, G.

Notes: Y-chromosome haplotypes of 150 males from 10 regions in Italy were determined using the PowerPlex® Y System and the ABI PRISM®3100 genetic analyzer. DNA samples were isolated from blood using the DNA IQ™ System. The total number of haplotypes oberved was 141, and 146 of the haplotypes were unique. Ten samples showed locus duplication. (3686)

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Forensic Sci. Int. 152, 89–94. Y-chromosomal STR haplotypes in a Belgian population sample and identification of a micro-variant with a flanking site mutation at DYS19. 2007

De Maesschalck, K,. Vanhoutte, E., Knaepen, K., Vanderheyden, N., Cassiman, J.J., and Decorte, R.

Notes: The authors collected DNA samples from 113 unrelated Belgian males. The PowerPlex® Y System and a GeneAmp® 9700 PCR system were used to amplify 12 Y-chromosome STR loci (DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439). Amplification products were detected using an ABI PRISM® 3100 genetic analyzer and POP-6™ polymer. Allele and haplotype frequencies and haplotype diversity were calculated. A total of 99 different haplotypes were observed. (3655)

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J. Forensic Sci. 51, 941–944. Allele frequencies and haplotypes of the 12 Y-STR loci using the PowerPlex Y system in Japanese population. 2006

Morikawa, T., Nakaki, S., Moriyoshi, H., Nakayama, H., Hino, D., Miyoshi, M. and Itohara, K.

Notes: The authors isolated DNA from 153 unrelated individuals fron the Hiroshima prefecture in Japan. Amplifications were carried out with 0.2–0.5ng of DNA and 10/20 cycling, and amplification products were detected using an ABI PRISM® 3100 genetic analyzer. Allele frequencies and gene diversity values are presented. (3644)

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Mol. Hum. Reprod. 12, 519–23. DAZ gene copies: evidence of Y chromosome evolution. 2006

Fernandes, A.T., Fernandes, S., Gonçalves, R., Sá, R., Costa, P., Rosa, A., Ferrás, C., Sousa, M., Brehm, A., and Barros, A.

Notes: The polymorphic DAZ gene family is located at the AZFc region of the human Y chromosome, and mutations or deletions within these genes can contribute to male infertility. The authors found an association between DAZ haplotypes and Y-STR haplotypes and used variation within 10 Y-STR loci to determine the coalescence time of DAZ haplotypes. The Y-STR haplotypes were determined using the PowerPlex® Y System. (3658)

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Int. Congr. Ser. 1288, 265–7. Haplotype analysis of the PowerPlex® Y System in Northeast population from Italy. 2006

Turrina, S., Atzei, R. and De Leo, D.

Notes: The authors generated population data and Y-STR haplotype frequencies for 105 unrelated males and 50 father-son pairs in northeast Italy. DNA was collected as blood or saliva samples, isolated then amplified using the PowerPlex® Y System as directed by the manufacturer. Amplified products were detected using an ABI PRISM® 3100-Avant Genetic Analyzer. (3867)

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Forensic Sci. Int. 158, 213–218. Y-chromosome STR haplotypes in a population sample from Switzerland (Zurich area). 2006

Haas, C., Wangensteen, T., Giezendanner, N., Kratzer, A. and Bär, W.

Notes: The authors collected buccal swabs from 150 unrelated Swiss males and isolated DNA for use in determination of haplotype frequencies of 12 Y-STR loci (DYS19, DYS385a/b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439). Purified DNA was amplified using the PowerPlex® Y System and a total of 30 cycles, and amplification products were detected using an ABI PRISM® genetic analyzer. Allele frequencies were calculated by direct counting. (3647)

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Forensic Sci. Int. 148, 225–231. Allele frequencies and haplotypes of the STR loci of the PowerPlex Y-system in southern populations from Korea. 2005

Chun, B.W., Shin, S.C., Kim, Y.J., Kim, K.S., Choi, D.H., Kim, K.H., Kim, J.Y. and Kang, H.S.

Notes: These authors used the PowerPlex® Y System to characterize Y-STR haplotype frequencies in southern populations in Korea. Blood and buccal swabs were collected from 259 Korean males, and DNA was isolated with the QIAamp DNA Mini Kit (Qiagen). The authors amplified 5–10ng of each sample (much more than the recommended 0.5–1.0ng) and detected the amplification products using an ABI PRISM® 310 genetic analyzer. (3637)

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Forensic Sci. Int. 149, 109–13. Genetic variability of 16 Y-chromosome STRs in a sample from Equatorial Guinea (Central Africa). 2005

Arroyo-Pardo, E., Gusmão, L., López-Parra, A.M., Baeza, C., Mesa, M.S. and Amorim, A.

Notes: The authors collected DNA samples from 101 donors from Equitorial Guinea. The Y-STR haplotypes at 12 loci were determined using the PowerPlex® Y System (DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439). Amplification products were detected using an ABI PRISM® 310 genetic analyzer. The haplotypes of five other loci (DYS460, DYS461, GATA A10, GATA C4 and GATA H4) were also determined. Haplotype and alelle frequencies and haplotype diversity were calculated. A total of 94 haplotypes were obtained.
(3653)

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Forensic Sci. Int. 150, 1–15. Twelve short tandem repeat loci Y chromosome haplotypes: genetic analysis on populations residing in North America. 2005

Budowle, B., Adamowicz, M., Aranda, X.G., Barna, C., Chakraborty, R., Cheswick, D., Dafoe, B., Eisenberg, A., Frappier, R., Gross, A.M., Ladd, C., Lee, H.S., Milne, S.C., Meyers, C., Prinz, M., Richard, M.L., Saldanha, G., Tierney, A.A., Viculis, L. and Krenke, B.E.

Notes: The Y-STR haplotypes of 2443 male individuals in North America were determined, and allele frequencies were compiled for African-Americans, Asians, Caucasians, Hispanics and Native Americans. The information presented includes haplotype frequencies, mutation rates, genetic diversity, population substructure effects and concordance. Y-STR haplotypes were determined using the PowerPlex® Y System. DNA samples were obtained as liquid whole blood, buccal swabs or dried blood on FTA® blood cards and extracted using phenol-chloroform extraction, a salting out method, Chelex®, the DNA IQ™ System or the QIAamp blood kit. Amplifications contained 0.5–1.0ng of purified DNA or a 1.2mm FTA® punch. Amplification products were detected using the ABI PRISM® 377 DNA sequencer or the ABI PRISM® 310 or 3100 genetic analyzer. (3638)

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Forensic Sci. Int. 151, 111–24. Validation of male-specific, 12-locus fluorescent short tandem repeat (STR) multiplex. 2005

Krenke, B.E., Viculis, L., Richard, M.L., Prinz, M., Milne, S.C., Ladd, C., Gross, A.M., Gornall. T., Frappier, J.R., Eisenberg, A.J., Barna, C., Aranda, X.G., Adamowicz, M.S., and Budowle, B.

Notes: The authors present the results of 17 developmental validation studies for the PowerPlex® Y System, which amplifies 12 Y-STR loci: DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439. The authors examined the effect of DNA quality and quantity, as well as analysis variation on the system. Variation of the amplification conditions, including cycle number, reaction volume, DNA polymerase concentration, magnesium concentration, annealing temperature and thermal cycler used, was also examined. The average stutter, specificity, precision and limitations of the system were also reported. Finally, the results demonstrated that the protocol performed as described. (3656)

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J. Forensic Sci. 50, 239—41. Y-chromosomal haplotypes for the PowerPlex Y for twelve STRs in a Peruvian population sample. 2005

Iannacone, G.C., Tito, R.Y., Lopez, P.W., Medina, M.E., and Lizarraga, B.

Notes: The authors collected blood samples, stored on FTA® blood cards, from 79 unrelated Peruvian males and isolated DNA using an unspecified method. The purified DNA was amplified using the PowerPlex® Y System and a Mastercycler gradient thermal cycler (Eppendorf). Amplified products were detected using an ABI PRISM® 310 genetic analyzer. Allele frequencies and gene diversity were calculated. A total of 76 different haplotypes were observed. (3657)

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Forensic Sci. Int. 152, 95–99. Y-chromosomal STR haplotypes in three ethnic groups and one cosmopolitan population from Tunisia. 2005

Cherni, L., Pereira, L., Goios, A., Loueslati, B.Y., Khodjet el Khil, H., Gomes, I., Gusmão, L., Alves, C., Slama, A., Amorim, A. and Elgaaied, A.B.

Notes: The authors collected DNA from 247 unrelated males in three ethnic groups in Tunisia to determine Y-STR haplotype frequencies. DNA was amplified using the PowerPlex® Y System, and amplified products were detected using an ABI PRISM® 310 genetic analyzer. The authors compared haplotype frequencies for 12 Y-STR loci (DYS19, DYS385a/b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439) in these three ethnic groups to other nearby populations. (3646)

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Forensic Sci. Int. 155, 205–210. Y-chromosome STR haplotypes in Danes. 2005

Hallenberg, C., Nielsen, K., Simonsen, B., Sanchez, J. and Morling, N.

Notes: These authors collected blood samples from 185 Danish males and (as negative controls) 11 females. Purified DNA was amplified using the PowerPlex® Y System and 10/20 cycling. Amplification products were detected using an ABI PRISM® 3100 genetic analyzer. A total of 163 Y-STR haplotypes were observed, including 144 unique haplotypes. For the DYS392 locus, a 10.2 allele was observed. All female DNA samples failed to amplify. (3648)

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Forensic Sci. Int. 151, 317–321. Y-chromosome STR haplotypes in Somalis. 2005

Hallenberg, C., Simonsen, B., Sanchez, J. and Morling, N.

Notes: The authors collected blood samples from 201 male Somalis to determine haplotype frequencies for 12 Y-STR loci (DYS19, DYS385a/b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439). Female DNA was tested as a negative control. Purified DNA (0.7µl of extracted DNA) or a 1.1mm2 FTA® blood card punch was amplified using the PowerPlex® Y System and 10/16 cycling. Amplification products were detected using a ABI PRISM® 3100 genetic analyzer. A total of 96 haplotypes were observed, 71 of which were unique. (3649)

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Forensic Sci. Int. 146 Suppl., S129–S131. Genetic study of 11 Y-STRs in the populations of Reggio Calabria, Catanzaro, Cosenza (Calabria--South of Italy). 2004

Barbaro, A., Cormaci, P., Falcone, G., Barbaro, A. and Rizzo, M.

Notes: Blood samples were collected from approximately 300 males in southern Italy. DNA was extracted, quantified using the AluQuant® Human DNA Quantitation System, and amplified using the PowerPlex® Y System. Amplification products were detected using the ABI PRISM® 310 genetic analyzer. The distribution of Y-STR haplotypes in three southern Italian populations was determined. (3639)

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