Promega Corporation

Apo-ONE™ Homogeneous Caspase3/7 Assay: Robust High-Throughput Apoptosis Detection

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Abstract

The biological importance of the apoptotic process has gained increased attention in recent years, ranging from general to specific analysis and encompassing fields of research as diverse as developmental biology and toxicology. However, limitations in a number of experimental methods for apoptosis detection have led to a compromise between apoptotic assay information content, complexity, throughput and rate of technical success. The advent of caspase-based proteolysis assays has opened the door to rapid and definitive confirmation of apoptotic events. Here we describe a simple, sensitive and flexible assay that is as robust and informative when automated with state-of-the-art robotics as it is when performed manually at the benchtop.

Promega Notes 81, 6–8.

Andrew Niles

Promega Corporation
Publication Date: 2002

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