GDNF E_max® Immunoassay System Technical Bulletin

Literature # TB221

The GDNF E_max® ImmunoAssay System is designed for the sensitive and specific detection of GDNF in an antibody sandwich format. In this format, 96-well plates are coated with Anti-GDNF Monoclonal Antibody (mAb), which binds soluble GDNF. The captured GDNF is bound by a second, specific polyclonal antibody (pAb). After washing, the amount of specifically bound pAb is detected using a species-specific antibody conjugated to horseradish peroxidase (HRP) as a tertiary reactant. The unbound conjugate is removed by washing, and following an incubation with a chromogenic substrate, the color change is measured. The amount of GDNF in the test solutions is proportional to the color generated in the oxidation-reduction reaction. Using this system, GDNF in tissue culture supernatants or tissue homogenates can be quantitated.

The GDNF E_max® ImmunoAssay System offers several benefits:

• Specificity: Specific detection of GDNF; typically less than 3% cross-reactivity with other neurotrophic factors at 10ng/ml.


• Sensitivity: Detects a minimum of 31.2pg/ml of GDNF.


• Flexibility: Available in sizes for two or five 96-well ELISA
  plates; can configure plates as desired.


• High Value: Optimized reagents and protocol.