The isolation of plasmid DNA from yeast can be more difficult than from bacterial cells due to the yeast cell wall and lower plasmid copy number. In this article, we demonstrate the isolation of plasmid from yeast using a one-step modification of the silica-membrane technology Wizard® Plus SV Minipreps DNA Purification System protocol.
Promega Notes 87, 27–28.
Vanesa G. Pannunzio1, Hilda I. Burgos1, Manuel Alonso1, Eugenia H. Ramos1, James R. Mattoon2, and Carlos A. Stella1