The TnT® Coupled Reticulocyte Lysate Systems offer a one-tube, coupled transcription/translation approach to protein expression. These systems reduce the time required to obtain in vitro translation results by incorporating transcription directly in the translation mix. There are several factors to be considered when choosing a TnT® System. These include the starting template, the type of protein to be translated, the preferred detection method, the desired reaction format and the intended downstream applications. Here we discuss how to decide which TnT® System is best for you.
Promega Notes 88, 24–26.