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pGEM® Express Control Template was transcribed using the RiboProbe® System—T7 following the instructions in Technical Manual #TM016. All reactions were performed in duplicate. Expected sizes in bases are displayed on the right of the gel image, and marker sizes in kb are listed on the left. The graph was generated by quantitating the bands on the gel and calculating a percent RNA compared to the positive control (polymerase + template). Error bars represent the two separate reactions. The black bars are quantitation of the top bands (2,346 bases) and the grey bars are quantitation of the bottom bands (1,065 bases).
Kanamycin Control RNA was reverse transcribed and amplified using the Plexor® One-Step qRT-PCR System with the addition of either Recombinant RNasin® Inhibitor or RNaseOUT™ Inhibitor. See Full Experiment
We analyzed samples with varying concentrations of Recombinant RNasin® Ribonuclease Inhibitor before and after six weeks of storage with repeated freeze-thaw cycles. Panel A. Represents samples before storage. Panel B. Represents samples after the six-week period. The gel models demonstrate that the samples show little RNA degradation.