Promega Corporation

Quality Reverse Transcriptases for First Strand cDNA Synthesis and RT-PCR

single-tube

M-MLV Rnase H Minus, Point Mutant Reverse transcriptase for routine first strand cDNA synthesis and end-point RT-PCR. Equivalent enzyme to SuperScript® II, but at a lower price.

single-tube

GoScript™ Reverse Transcriptase Robust and sensitive reverse transcriptase optimized for RT-qPCR applications. Exceptional performance on long or rare transcripts.

single-tube

AMV Reverse Transcriptase Reverse transcriptase well suited for templates with a high degree of secondary structure.

Promega Reverse Transcriptases at a Glance

AMV Reverse Transcriptase M-MLV Reverse Transcriptase, RNase H Minus Point Mutant GoScript™ Reverse Transcriptase
Target Applications
  • RT-PCR templates with high secondary structure
  • Routine cDNA synthesis
  • RT-PCR
  • Wide range of target abundance (low to high)
  • Reverse transcriptase when there is a possibility of PCR inhibitors present
  • RT-qPCR
General Reaction Temperature 42°C 42°C 37°C
Reduced RNase H Activity Yes
RT-PCR Kit Format Yes Yes
Ordering Information Ordering Information Ordering Information
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