Tth DNA Polymerase

Tth DNA Polymerase is a thermostable enzyme of approximately 94kDa isolated from Thermus thermophilus HB-8. The enzyme replicates DNA at 74°C and exhibits a half-life of 20 minutes at 95°C. Tth DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium and the polymerization of nucleotides into DNA using an RNA template in the 5´→3´ direction in the presence of manganese. The enzyme also possesses a 5´→3´ exonuclease activity. Tth DNA Polymerase is recommended for use in PCR, RT-PCR, reverse transcription and primer extension reactions at ...

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Tth DNA Polymerase is a thermostable enzyme of approximately 94kDa isolated from Thermus thermophilus HB-8. The enzyme replicates DNA at 74°C and exhibits a half-life of 20 minutes at 95°C. Tth DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium and the polymerization of nucleotides into DNA using an RNA template in the 5´→3´ direction in the presence of manganese. The enzyme also possesses a 5´→3´ exonuclease activity. Tth DNA Polymerase is recommended for use in PCR, RT-PCR, reverse transcription and primer extension reactions at elevated temperatures.

10X Reverse Transcription Buffer: 100mM Tris-HCl (pH 8.3 at 25°C), 900mM KCl.

10X Chelate Buffer: 100mM Tris-HCl (pH 8.3 at 25°C), 1M KCl, 7.5mM EGTA, 0.5% Tween® 20, 50% glycerol.

Thermophilic DNA Polymerase 10X Reaction Buffer: 500mM KCl, 100mM Tris-HCl (pH 9.0 at 25°C) and 1% Triton® X-100. Buffer is optimized for use with 0.2mM of each of the dNTPs.

Manganese and Magnesium Chloride: 10mM MnCl₂ and 25mM MgCl₂ Solutions provided.

Features - Benefits

• Increased Specificity for RT-PCR: The ability to reverse transcribe at higher temperatures results in increased specificity of primer hybridization and extension.
• Minimized Secondary Structures: Higher temperature RT-PCR minimizes problems associated with strong secondary structures in RNA.
• Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.

Applications

• RT-PCR.
• PCR.
• Primer extension.
• Reverse transcription.

For more information, see the Protocols & Applications Guide.

References

1. Ruttimann, C. et al. (1985) Eur. J. Biochem. 149, 41–6.

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