GoTaq® MDx Hot Start Polymerase

GoTaq® MDx Hot Start Polymerase contains the high-performance GoTaq® DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The polymerase activity is restored during the initial denaturation step when the amplification reactions are heated at 94–95°C for two minutes. The system is supplied with a tube of 25mM MgCl₂, allowing optimization of the magnesium concentration in your reactions. It is also supplied with 5X Green GoTaq® Flexi Buffer and 5X Colorless GoTaq® Flexi Buffer. The buffers contain a compound that increases sample density, so that samples sink easily into the wells of an ag...

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GoTaq® MDx Hot Start Polymerase contains the high-performance GoTaq® DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The polymerase activity is restored during the initial denaturation step when the amplification reactions are heated at 94–95°C for two minutes. The system is supplied with a tube of 25mM MgCl₂, allowing optimization of the magnesium concentration in your reactions. It is also supplied with 5X Green GoTaq® Flexi Buffer and 5X Colorless GoTaq® Flexi Buffer. The buffers contain a compound that increases sample density, so that samples sink easily into the wells of an agarose gel. The green buffer also contains two dyes (yellow and blue) that separate to allow easy monitoring during electrophoresis. Use the green reaction buffer for direct-to-gel analysis after amplification and the colorless reaction buffer for amplifications where the dyes may interfere with post-amplification analysis such as fluorescence or absorbance testing.

GoTaq® MDx Hot Start Polymerase is a general purpose reagent that can be used for clinical applications or as a component of molecular diagnostic assays without paying royalties. The products by themselves do not provide any diagnostic result.

Features - Benefits

The proven robust amplification of GoTaq® MDx Hot Start Polymerase is now available for use in diagnostics and clinical applications:

• Easy: Convenient handling with room temperature setup.
• Fast: Results with only two-minute enzyme activation.
• Enhanced: Yield, sensitivity and specificity with an antibody hot start.
• Ready: High-quality product developed for integration into Laboratory Developed Tests and homebrew assays.

To use GoTaq® MDx Hot Start Polymerase in a custom format for diagnostic assays or to distribute GoTaq® MDx Hot Start Polymerase, please contact the Promega Custom Order Department to discuss specific requirements.

Applications

For use in molecular diagnostics, laboratory designed tests (LDTs) and homebrew assays.

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