MagneGST™ Pull-Down System

V8870o
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MagneGST™ Pull-Down System

The MagneGST™ Pull-Down System is designed to detect protein interactions between GST-fusion proteins expressed in bacterial lysates and prey proteins expressed in the TnT® Systems. Prey protein synthesized in the TnT® Quick Coupled Transcription/Translation Reaction is captured using bait protein (GST-fusion protein) immobilized on MagneGST™ Particles. Nonspecifically bound proteins are washed away, and the prey protein is analyzed. Prey proteins can be detected by incorporating radioactively labeled methionine in the TnT® Quick reaction, followed by SDS-PAGE and autoradiography or by incorporating the supplied non-radioactive methionine in the TnT® reaction and detecting by Western blotting with protein-specific antibodies (see figure).

Note: This product requires the use of a magnetic stand, which is not included.

For more information, see the Protocols & Applications Guide.

Application

  • GST pull-down assays for the analysis of protein:protein interactions.
Representation of pull-down assay using bacterial expression of bait protein and TNT® cell-free system for the expression of the prey protein.

Representation of pull-down assay using bacterial expression of bait protein and TnT® cell-free system for the expression of the prey protein.

Specifications

What's in the box?

Item Part # Size Concentration Available Separately

Methionine

L118A 1 × 50μl 1mM

RQ1 RNase-Free DNase

M610A 1 × 1,000u View Product

Nuclease-Free Water

P119A 1 × 1,250μl View Product

MagneGST™ Cell Lysis Reagent

V481B 1 × 60ml

MagneGST™ Glutathione Particles

V861A 1 × 4ml View Product

MagneGST™ Binding/Wash Buffer

V870B 2 × 200ml

TnT® T7 Quick Master Mix

L520A 8 × 200μl

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

 

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